Virus induced gene silencing (VIGS) for functional analysis of wheat genes involved in Zymoseptoria tritici susceptibility and resistance

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Virus induced gene silencing (VIGS) for functional analysis of wheat genes involved in Zymoseptoria tritici susceptibility and resistance

Virus-induced gene silencing (VIGS) has emerged as a powerful reverse genetic technology in plants supplementary to stable transgenic RNAi and, in certain species, as a viable alternative approach for gene functional analysis. The RNA virus Barley stripe mosaic virus (BSMV) was developed as a VIGS vector in the early 2000s and since then it has been used to study the function of wheat genes. Se...

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Quantitative expression analysis of candidate genes for Septoria tritici blotch resistance in wheat (Triticum aestivum L.)

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tomato and tobacco phytoene desaturase gene silencing by virus-induced gene silencing (vigs) technique

background and aims: virus-induced gene silencing (vigs) is a virus vector technology that exploits antiviral defense mechanism. by infecting plants with recombinant viruses containing host genes inserted in the viral genome, vigs achieves the rna silencing process. the purpose of this study was to investigate the possibility of tomato (lycopersicon esculentum mill.) and tobacco (nicotiana bent...

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ژورنال

عنوان ژورنال: Fungal Genetics and Biology

سال: 2015

ISSN: 1087-1845

DOI: 10.1016/j.fgb.2015.04.006